PCB降糖片的纯化工艺及体外药效研究

PCB降糖片的纯化工艺及体外药效研究(论文7500字)
内容摘要：研究大孔树脂精制PCB醇提液中总黄酮，水提液中多酚、多糖的工艺条件。方法：把PCB的总黄酮吸附率和解析率作为指标，研究4种型号完全不同的大孔树脂对PCB总黄酮的吸附力及与解析力，确认D101作为最适树脂。优化D101大孔树脂的纯化条件；将PCB多酚、多糖吸附率与解析率作为指标，研究5种不同类型大孔树脂对PCB多酚、多糖的吸附力和解析力，将AB-8作为最适树脂。优化AB-8大孔树脂纯化条件。结果：PCB总黄酮分离富集条件为:上样速度2 BV/h，上样量120.00 mL，上样浓度为4.890 mg/mL，吸附1.5 h，除杂溶剂4%乙醇，除杂速度3 BV/h，用量256.41 mL，将60%乙醇作为洗脱溶剂，3 BV/h的洗脱速度，125.00 mL洗脱剂用量，经D101 处理后PCB总黄酮纯度至55. 25% ；多酚、多糖的分离富集条件为:上样速度2 BV/h，上样量15.00 mL，上样浓度为0.5 g/mL（生药/溶剂），1.5 h吸附，40%乙醇作为洗脱溶剂，3 BV/h的洗脱速度，90.00 mL洗脱剂用量，经AB-8处理后的PCB多酚、多糖纯度为10.76%、40.92%，结论：D101型的大孔吸附树脂能较好将PCB总黄酮纯化富集；AB-8型大孔树脂能较好将PCB多酚、多糖纯化富集。

关键词：PCB 降糖　纯化工艺
 
Study on purification technology and in vitro efficacy of PCB Jiangtang tablets
Abstract: the research of macroresin PCB ethanol extract of total flavonoids in liquid water extraction of polyphenols, process conditions of polysaccharides. Methods: the total flavone adsorption rate and resolution rate of PCB as index, adsorption capacity of macroporous resin on the 4 types of different PCB total flavonoids and resolution D101, recognized as the optimum resin. Optimum purification conditions of D101 macroporous resin; PCB polyphenols, rate and desorption rate as the index of adsorption of polysaccharide, 5 different types of macroporous resin on PCB adsorption capacity and resolution of polyphenols, polysaccharides, AB-8 as the optimum resin. The optimized AB-8 Kong Shuzhi purification conditionResults: PCB flavonoids separation and enrichment conditions were the sample rate of 2 BV/h, sample volume of 120 mL, the concentration of sample was 4.890 mg/mL, 1.5 h of adsorption, impurity removal rate of solvent was 4% ethanol, 3 BV/h, 256.41 mL dosage, 60% ethanol as elution solvent, elution rate of 3 BV/h, 125 mL eluting agent dosage, after treatment with D101 PCB to 55.25% purity of total flavonoids; polyphenol, polysaccharide separation and enrichment conditions were the sample rate of 2 BV/h, sample volume 15ML, the concentration of sample was 0.5 g/mL (crude drug / solvent), 1.5 h adsorption, 40% ethanol as elution solvent, elution rate of 3 BV/h, 90 mL, eluent dosage, polyphenol by PCB after AB-8 treatment, the purity of polysaccharide was 10.76%, 40.92%, conclusion: D101 macroporous resin can be used to the purification of total flavonoids from PCB enrichment; AB-8 macroporous resin could be PCB polyphenol, polysaccharide separation and purification.

Key words: PCB hypoglycemic purification process

目录
内容摘要：    I
引 言    III
一、实验材料    1
（一）、实验药品和试剂    1
（二）、实验器材    1
（三）、实验动物和材料    2
二、方法与结果    2
（一）、总黄酮纯化工艺研究    2
（二）多糖、多酚纯化工艺的研究    8
（三）、细胞试验    13
三、结论    14
参考文献    16