厌氧甲烷氧化反硝化的强化研究
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厌氧甲烷氧化反硝化的强化研究(任务书,开题报告,外文翻译,论文10000字)
摘要
微生物进行的厌氧甲烷氧化反硝化过程是减少自然环境中甲烷这一温室气体排放的重要生物途径,即厌氧型甲烷氧化反硝化(denitrifying anaerobic methane oxidation,DAMO)。该过程能够去除硝酸盐氮,并控制温室气体甲烷,具有潜在的研究与应用价值。但厌氧甲烷氧化反硝化效率较低,微生物较为敏感,本文通过添加活性炭、生物炭、固态腐殖质等自然界中分布广泛的电子媒介体,研究其对厌氧甲烷氧化反硝化的影响。结果表明,活性炭对于甲烷厌氧氧化反硝化(DAMO)过程有一定的促进作用,分析原因可能是活性炭促进了甲烷氧化与反硝化微生物之间的电子传递,需要进一步深层次的机理分析和实验验证。生物炭和腐殖质对于DAMO过程没有比较明显的影响。
关键词:反硝化型甲烷厌氧氧化(DAMO);活性炭;生物炭
Abstract
The anaerobic methane oxidation and denitrification process by microorganisms is an important biological pathway to reduce the greenhouse gas emissions of methane in the natural environment,namely anaerobic methane oxidation (DAMO). This process has the potential for research and application by removing nitrate nitrogen and controlling greenhouse gas methane. However,the anaerobic methane oxidation denitrification efficiency is low, and the microorganisms are sensitive. In this paper, the effects of anaerobic methane oxidation and denitrification on the anaerobic methane oxidation denitrification are studied by adding widely distributed electronic media such as activated carbon,biochar and solid humus. The results show that activated carbon can promote the process of methane anaerobic oxidation denitrification (DAMO). The overall effect is that its conduction promotion or adsorption is still unknown due to the limited author level. Further deep mechanism is needed. Analysis and experimental verification. Biochar and humin has basically no obvious influence on the DAMO process.
Key words: denitrification methane anaerobic oxidation (DAMO); activated carbon; biochar
目录
摘要 1
Abstract 1
第一章绪论 1
1.1研究背景 1
1.2研究目的与意义 2
第二章反应机理及研究进展 4
2.1反应机理简述 4
2.2研究进展 5
第三章材料与方法 7
3.1 接种物 7
3.2 培养基及培养体系等溶液 7
3.3 培养装置 8
3.4计算甲烷在培养体系中的溶解度 9
3.5 离子测定方法 10
3.6 实验步骤 12
第四章数据分析与结果讨论 14
4.1离子浓度的计算方法 14
4.2一轮实验具体结果展示 14
4.3 二轮实验具体结果 21
第五章结论 28
参考文献 29
致谢 31